Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types.
Abstract
A medium is described that supports long-term growth in culture of human primary mammary tumor cells, of normal epithelial cells from mammoplasty, and of mammary tumor cell lines. Tumor cells are shown to be distinguishable from normal mammary epithelial cells by morphology, by growth requirements, and by two markers: preferential expression of the HMFG-2 epitope on tumor cells and preferential retention in tumor cell mitochondria of the lipophilic fluorescent dye rhodamine 123. Differential fluorescence of HMFG-2 fluorescein-conjugated antibodies can be used as a basis for separation of normal and tumor cells in a fluorescence-activated cell sorter, as can differential retention of rhodamine 123.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 해부 | mammary
|
유방 | dict | 4 | |
| 시술 | mammoplasty
|
유방성형술 | dict | 1 | |
| 해부 | cell
|
scispacy | 1 | ||
| 해부 | mammary tumor cells
|
scispacy | 1 | ||
| 해부 | normal epithelial cells
|
scispacy | 1 | ||
| 해부 | mammary tumor cell lines
|
scispacy | 1 | ||
| 해부 | Tumor cells
|
scispacy | 1 | ||
| 해부 | mammary epithelial cells
|
scispacy | 1 | ||
| 해부 | tumor cell
|
scispacy | 1 | ||
| 약물 | rhodamine
|
C0600322
Rhodamine
|
scispacy | 1 | |
| 약물 | lipophilic fluorescent dye rhodamine
|
scispacy | 1 | ||
| 질환 | human primary mammary tumor
|
scispacy | 1 | ||
| 질환 | tumor
|
C0027651
Neoplasms
|
scispacy | 1 | |
| 기타 | human mammary epithelial cells
|
scispacy | 1 | ||
| 기타 | human
|
scispacy | 1 | ||
| 기타 | HMFG-2
|
scispacy | 1 |
MeSH Terms
Breast; Breast Neoplasms; Cell Division; Cells, Cultured; Culture Media; Culture Techniques; DNA; DNA, Neoplasm; Epithelial Cells; Female; Flow Cytometry; Humans; Tumor Cells, Cultured
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