A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications.
Abstract
White adipose tissue is the most abundant and accessible source of stem cells in the adult human body. In this paper, we present a standardised and safe method of isolating and maximizing the number of adipose-derived stem cells (ASCs) from conventional liposuction for clinical applications, which was carried out through both mechanical (centrifuge) and enzymatic (collagenase) means. Isolated cells were characterized through flow cytometry assay. Gathered data showed a greater amount (9.06 × 10(5) ASCs from 100 mL of adipose tissue) of isolated ASCs compared to previous protocol, also with high (99%) cell vitality; the procedure we presented is easy and fast (80 minutes), allowing collecting a significative number of mesenchymal stem cells, which can be used for clinical purposes, such as wound healing.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 시술 | liposuction
|
지방흡입 | dict | 1 | |
| 해부 | Adipose-Derived Stem Cells
|
scispacy | 1 | ||
| 해부 | adipose tissue
|
scispacy | 1 | ||
| 해부 | stem cells
|
scispacy | 1 | ||
| 해부 | ASCs
→ adipose-derived stem cells
|
scispacy | 1 | ||
| 해부 | cells
|
scispacy | 1 | ||
| 해부 | cell
|
scispacy | 1 | ||
| 해부 | mesenchymal stem cells
|
scispacy | 1 | ||
| 합병증 | wound
|
scispacy | 1 | ||
| 기타 | human body
|
scispacy | 1 | ||
| 기타 | collagenase
|
scispacy | 1 |
MeSH Terms
Adipose Tissue; Adult; Cell Separation; Cell Survival; Flow Cytometry; Humans; Lipectomy; Sensitivity and Specificity; Stem Cell Transplantation; Stem Cells
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