Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser.

Methods in molecular biology (Clifton, N.J.) 2022 Vol.2468() p. 319-328

Harreguy MB, Tran TS, Haspel G

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Abstract

Laser microsurgery allows the user to ablate cell bodies or disconnect nerve fibers by using a laser microbeam focused through a microscope. This technique was pioneered in C. elegans where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in C. elegans can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we describe how to assemble and operate a platform for Yb-doped fiber laser microsurgery. The novel laser setup described here is a more robust, lower cost, and user-friendly alternative to other femtosecond-pulsed laser systems.

추출된 의학 개체 (NER)

유형영어 표현한국어 / 풀이UMLS CUI출처등장
시술 microsurgery 미세수술 dict 3
해부 Neuronal scispacy 1
해부 cell scispacy 1
해부 neurons scispacy 1
해부 dendrites scispacy 1
해부 cellular scispacy 1
질환 neuroregeneration C4326926
Nerve Tissue Regeneration
scispacy 1
기타 nerve fibers scispacy 1

MeSH Terms

Animals; Caenorhabditis elegans; Lasers; Light; Microsurgery; Neurons

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