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10건 · 최신순-
Reconstituted normal human breast in nude mice: effect of host pregnancy environment and human chorionic gonadotropin on proliferation.
The proliferation of normal human breast epithelial cells in women is highest during the first trimester of pregnancy. In an attempt to analyze this hormonal environment in a model system, the effect of host mouse pregnancy and the administ…
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Estrogen and progesterone receptors can be maintained in normal human breast epithelial cells in primary culture and after transplantation into nude mice.
The estrogen and progesterone receptor expression was determined in breast epithelial cells from reduction mammoplasty specimens. Only a subset of the luminal epithelial cell population was found to be positive for these receptors. When the…
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Estrogen receptor variants in epithelial compartment of normal human breast.
Estrogen receptor (ER) splice variants have previously been identified in normal human breast. Normal breast, however, comprises many cell types including fat cells, fibroblasts, endothelial cells, and a variety of inflammatory cells beside…
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Adeno-associated virus vector mediated transduction of primary normal human breast epithelial cells.
Cultured human breast epithelial cells from reduction mammoplasty specimens were transduced using an adeno-associated virus vector encoding the marker gene E. coli -galactosidase. Subconfluent, growing, breast epithelial cells were more eas…
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Isolation of a cDNA for the human homolog of MAT-1 oncogene and its expression in human breast cancer cells.
Overexpression of mouse MAT-1 oncogene is associated with carcinogensis of mouse mammary epithelial cells. A human cDNA (hMAT) homologous to MAT-1 was isolated from normal human breast tissue. The hMAT cDNA is 1312 bp long and produces a 8.…
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In vivo growth stimulation of collagen gel embedded normal human and mouse primary mammary epithelial cells.
A new system for studying growth of normal human mammary epithelial cells in an in vivo environment using athymic nude mice is described. Human mammary epithelial cells dissociated from reduction mammoplasty specimens were embedded within c…
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Reconstituted human normal breast in nude mice using collagen gel or Matrigel.
Human breast epithelial cells dissociated from reduction mammoplasty specimens were embedded in two commonly used extracellular matrices, type I collagen gel or Matrigel, and subsequently transplanted subcutaneously into athymic nude mice. …
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Adenoviral-mediated gene transfer into primary human and mouse mammary epithelial cells in vitro and in vivo.
Primary mammary epithelial cells from both the human and mouse mammary glands can be genetically altered under a variety of situations using the replication-defective adenoviral vector containing a marker gene encoding the E. coli beta-gala…
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Depletion of O6-alkylguanine-DNA alkyltransferase by O6-benzylguanine in three-dimensional collagen cultures of normal human breast epithelial cells.
O6-Alkylguanine--DNA alkyltransferase (AGT) is a protein which removes the promutagenic O6-alkylguanine lesion induced in DNA by alkylating agents. Our results demonstrate that freshly isolated organoids from reduction mammoplasty specimens…
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Human breast epithelial cells in serum-free collagen gel primary culture: growth, morphological, and immunocytochemical analysis.
Human breast epithelial cells derived from various sources (fibroadenoma, reduction mammoplasty, and mastectomy tissues from premenopausal patients) have been cultured in collagen gel matrix using serum-free medium. Response to various addi…