In vivo construction of lymphoid node by implantation of adipose-derived stromal cells with hydroxypropyl methyl cellulose hydrogel in BALB/c nude mice.
Abstract
Adipose-derived stromal cells have multilineage potential to differentiate into several specialized tissue types. Herein, we investigated whether ADSCs could differentiate into lymphoid node . Human ADSCs from routine liposuction were cultured in differentiation medium and were supplemented with transforming growth factor β1 (TGF)-β1 and basic fibroblast growth factor (bFGF). The induced hADSCs mixed with 13% (w/v) hydroxypropyl methylcellulose (HPMC) were injected into BALB/c mice subcutaneously. Eight weeks later, nodules were found under the injected sites. Histology, immunohistochemistry, and species identification analysis confirmed that the nodules were lymphoid nodes that were derived from the injected hADSCs. Our experiment demonstrated that the hADSCs could differentiate into lymphocyte-like cells and form lymphoid nodes . TGF-β1 and bFGF might play important roles in the differentiation of hADSCs into lymphocyte-like cells. Our study might present an alternative approach for engineering immune organs and thus offer potential treatment for immunodeficiency diseases.
추출된 의학 개체 (NER)
| 유형 | 영어 표현 | 한국어 / 풀이 | UMLS CUI | 출처 | 등장 |
|---|---|---|---|---|---|
| 시술 | liposuction
|
지방흡입 | dict | 1 | |
| 해부 | adipose-derived stromal cells
|
scispacy | 1 | ||
| 해부 | tissue
|
scispacy | 1 | ||
| 해부 | ADSCs
|
scispacy | 1 | ||
| 해부 | hADSCs
|
scispacy | 1 | ||
| 해부 | lymphocyte-like cells
|
scispacy | 1 | ||
| 합병증 | lymphoid node
|
scispacy | 1 | ||
| 약물 | hydroxypropyl methyl cellulose
|
C0063242
hypromellose
|
scispacy | 1 | |
| 약물 | ADSCs
|
scispacy | 1 | ||
| 약물 | HPMC
→ hydroxypropyl methylcellulose
|
C0063242
hypromellose
|
scispacy | 1 | |
| 질환 | immunodeficiency diseases
|
C0021051
Immunologic Deficiency Syndromes
|
scispacy | 1 | |
| 질환 | nodules
|
scispacy | 1 | ||
| 질환 | nodules were lymphoid nodes
|
scispacy | 1 | ||
| 질환 | immune organs
|
scispacy | 1 | ||
| 기타 | BALB/c nude mice
|
scispacy | 1 | ||
| 기타 | lymphoid node
|
scispacy | 1 | ||
| 기타 | Human ADSCs
|
scispacy | 1 | ||
| 기타 | transforming growth factor β1 (TGF)-β1
|
scispacy | 1 | ||
| 기타 | basic fibroblast growth factor
|
scispacy | 1 | ||
| 기타 | bFGF
→ basic fibroblast growth factor
|
scispacy | 1 | ||
| 기타 | BALB/c mice
|
scispacy | 1 | ||
| 기타 | lymphoid nodes
|
scispacy | 1 |
MeSH Terms
Adipocytes; Adipose Tissue; Animals; Cell Differentiation; Cell Lineage; Cell Proliferation; Fibroblast Growth Factor 2; Humans; Hydrogels; Hypromellose Derivatives; Immunohistochemistry; Lymph Nodes; Lymphocytes; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Organ Culture Techniques; Phenotype; Stromal Cells; Tissue Engineering; Transforming Growth Factor beta
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