What Happened to Masticatory Muscles after Botulinum-Toxin-Type A Injection into Masseter: A Systematic Review.

Aesthetic plastic surgery 2026 Vol.50(3) p. 1320-1328

Yan M, Zhao M, Liu J, Luo Y, Qiu Y, Guo Z, Yu P, Lu J

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Abstract

[BACKGROUND] Botulinum toxin type A (BoNT-A) is commonly used in neuromuscular disorders and cosmetic fields, but the detailed histological and subcellular changes in masticatory muscles, mainly in masseter, remain underexplored.

[METHODS] The literature review was conducted using the PubMed and Cochrane databases between January 2000 and September 2024. Studies of BoNT-A injection in masseters with available full- text and extractable data were included and were grouped depending on injected species. The sample size, dose of injection, follow-up duration, and detailed muscle changes were concluded.

[RESULTS] Fourteen articles were eligible for reviewing, among which 2 were conducted on humans, and 12 were conducted on animals including rats (n=5), mice (n=2), rabbits (n=3) and pigs (n=2). Morphological analysis showed muscle atrophy with variable recovery. Histological findings included decreased fiber diameter, disrupted arrangements, and shifts in myosin-heavy-chain fiber types. Immunohistochemistry indicated increased cell proliferation and nuclear centralization. Electrophysiological assessments demonstrated reduced EMG amplitudes and shorter signal durations, correlating with muscle size and bite force. Ion analysis revealed increased sodium, chloride and sulfur, and decreased potassium and phosphorus.

[CONCLUSION] BoNT-A injection causes dose-dependent denervation and dysfunction in masticatory muscles, with incomplete recovery over time. However, due to very limited human data, these findings should be interpreted cautiously, and strong clinical extrapolations are not justified. These findings provide valuable insights into the long-term effects of BoNT-A on muscle structure and function, highlighting the need for further research into the mechanisms underlying these changes and their clinical implications for both therapeutic and aesthetic applications.

[LEVEL OF EVIDENCE III] This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

추출된 의학 개체 (NER)

유형영어 표현한국어 / 풀이UMLS CUI출처등장
시술 botulinum toxin 보툴리눔독소 주사 dict 1
해부 Masseter: A Systematic Review. scispacy 1
해부 neuromuscular scispacy 1
해부 masseter scispacy 1
해부 masseters scispacy 1
해부 muscle scispacy 1
해부 cell scispacy 1
해부 nuclear scispacy 1
해부 Ion scispacy 1
합병증 masticatory muscles scispacy 1
합병증 EMG scispacy 1
약물 sodium C0037473
sodium
scispacy 1
약물 chloride C0008203
Chlorides
scispacy 1
약물 sulfur C0038774
sulfur
scispacy 1
약물 potassium C0032821
potassium
scispacy 1
약물 phosphorus C0031705
phosphorus
scispacy 1
약물 [BACKGROUND] Botulinum toxin type A scispacy 1
약물 BoNT-A → Botulinum toxin type A scispacy 1
질환 neuromuscular disorders C0027868
Neuromuscular Diseases
scispacy 1
질환 muscle atrophy C0026846
Muscular Atrophy
scispacy 1
질환 Masticatory Muscles scispacy 1
기타 BoNT-A → Botulinum toxin type A scispacy 1
기타 humans scispacy 1
기타 rats scispacy 1
기타 mice scispacy 1
기타 rabbits scispacy 1
기타 pigs scispacy 1
기타 myosin-heavy-chain fiber scispacy 1
기타 human scispacy 1

MeSH Terms

Botulinum Toxins, Type A; Animals; Masseter Muscle; Humans; Injections, Intramuscular; Neuromuscular Agents; Rabbits; Mice; Muscular Atrophy; Rats

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