Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons.
💡 한 문장 핵심
다능성 nestin 양성, keratin 음성 모낭 bulge 줄기세포는 신경세포로 분화할 수 있다.
5/5 보강
TL;DR
It is demonstrated that ND-GFP stem cells isolated from the hair-follicle bulge area that are negative for the keratinocyte marker keratin 15 can differentiate into neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro.
📑 코퍼스 인용 관계
· 인용됨 54 · 인용함 16
→ 이 논문이 인용한 논문 (2) ▾
- Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells. Proceedings of the National Academy of Sciences of the United States of America · 2004
- Morphogenesis and renewal of hair follicles from adult multipotent stem cells. Cell · 2001
📑 인용한 논문 (6) ▾
- Melatonin-Mediated Circadian Rhythm Signaling Exhibits Bidirectional Regulatory Effects on… Biomolecules · 2025
- Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestio… International journal of molecular sciences · 2025
- LncRNA RP11-818O24.3 regulates proliferation and differentiation of hair follicle stem cel… PloS one · 2025
- Rat hair-follicle-associated pluripotent (HAP) stem cells can differentiate into atrial or… PloS one · 2024
- Hair-follicle associated pluripotent (HAP)-cell-sheet implantation enhanced wound healing … PloS one · 2024
- In Vitro differentiation of hair-follicle bulge stem cells into synaptophysin-expressing n… Human cell · 2024
연도별 인용 (2012–2026) · 합계 243
OpenAlex 토픽 ·
Hair Growth and Disorders
Neurogenesis and neuroplasticity mechanisms
Nerve injury and regeneration
🇰🇷 한글 요약 🌐 Abstract
【연구 목적】
모낭 융기부(hair-follicle bulge area)에 존재하는 nestin 양성·keratin 15 음성 줄기세포가 신경세포를 비롯한 다양한 세포로 분화할 수 있는 다분화능(multipotency)을 지니는지 검증하고자 함.
【방법】
Nestin 조절요소에 의해 GFP가 발현되는 형질전환 마우스(ND-GFP)를 이용하여 모낭 융기부에서 nestin 양성·keratin 15 음성 줄기세포를 분리하였고, in vitro 분화 유도 및 누드마우스 피하 이식(subcutis transplantation)을 통해 분화능을 평가함.
【주요 결과】
분리된 ND-GFP 줄기세포는 CD34 양성·keratin 15 음성의 미분화 상태를 보였으며, in vitro에서 신경세포(neuron), 신경아교세포(glia), 각질형성세포(keratinocyte), 평활근세포(smooth muscle cell), 멜라닌세포(melanocyte)로 분화하였고, 누드마우스 피하 이식 후에도 신경세포로 분화함이 확인됨.
【임상적 시사점 (성형외과 의사 관점)】
환자의 두피 모낭에서 비교적 간단히 채취 가능한 자가(autologous) 다분화능 줄기세포원으로 활용 가능성이 있어, 안면신경 손상 후 신경 재생, 흉터·결손 부위의 피부·평활근·멜라닌세포 재건, 모발 이식 및 재생 치료 등 성형·재건 영역에서 미래 세포치료 자원으로 응용될 수 있음.
We have recently shown that the expression of nestin, the neural stem cell marker protein, is expressed in bulge-area stem cells of the hair follicle. We used transgenic mice with GFP expression driven by the nestin regulatory element [nestin-driven GFP (ND-GFP)]. The ND-GFP stem cells give rise to the outer-root sheath of the hair follicle as well as an ND-GFP interfollicular vascular network. In this study, we demonstrate that ND-GFP stem cells isolated from the hair-follicle bulge area that are negative for the keratinocyte marker keratin 15 can differentiate into neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. These pluripotent ND-GFP stem cells are positive for the stem cell marker CD34, as well as keratin 15-negative, suggesting their relatively undifferentiated state. The apparent primitive state of the ND-GFP stem cells is compatible with their pluripotency. Furthermore, we show that cells derived from ND-GFP stem cells can differentiate into neurons after transplantation to the subcutis of nude mice. These results suggest that hair-follicle bulge-area ND-GFP stem cells may provide an accessible, autologous source of undifferentiated multipotent stem cells for therapeutic application.
【연구 목적】 모낭 융기부(hair-follicle bulge area)에 존재하는 nestin 양성·keratin 15 음성 줄기세포가 신경세포를 비롯한 다양한 세포로 분화할 수 있는 다분화능(multipotency)을 지니는지 검증하고자 함.
APA 7
Amoh, Y., Li, L., Katsuoka, K., Penman, S., & Hoffman, R. M. (2005). Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons.. Proceedings of the National Academy of Sciences of the United States of America, 102(15), 5530-4. https://doi.org/10.1073/pnas.0501263102
Vancouver
Amoh Y, Li L, Katsuoka K, Penman S, Hoffman RM. Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc. Nati. Acad. Scie. Unit. Stat. Amer.. 2005;102(15):5530-4. doi:10.1073/pnas.0501263102
AMA 11
Amoh Y, Li L, Katsuoka K, Penman S, Hoffman RM. Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons. Proc. Nati. Acad. Scie. Unit. Stat. Amer.. 2005;102(15):5530-4. doi:10.1073/pnas.0501263102
Chicago
Amoh, Y., Li, L., Katsuoka, K., Penman, S., and Hoffman, R. M.. 2005. "Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons." Proceedings of the National Academy of Sciences of the United States of America 102 (15): 5530-4. https://doi.org/10.1073/pnas.0501263102
MLA 9
Amoh, Y., et al. "Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons." Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 15, 2005, pp. 5530-4. doi:10.1073/pnas.0501263102.
PMID
15802470 ↗
🏷️ 키워드 / MeSH 📖 같은 키워드 OA만
인용 관계
그래프 OA 노드: 10/10 (100%)
· 참조 2편 · 후속 8편
이 논문이 참조한 문헌 16
이 논문을 인용한 후속 연구 20
- Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerv…
- From hair to heart: nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells differen…
- Recreation of a hair follicle regenerative microenvironment: Successes and pitfalls.
- Stem cells with neural crest characteristics derived from the bulge region of cultured human hair fo…
- Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitr…
- Hair follicle-associated-pluripotent (HAP) stem cells.
- Cryopreservation of the Hair Follicle Maintains Pluripotency of Nestin-Expressing Hair Follicle-Asso…
- Neural potential of a stem cell population in the hair follicle.
- Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury.
- Real-time confocal imaging of trafficking of nestin-expressing multipotent stem cells in mouse whisk…
- Hair Follicle Stem Cells for Tissue Regeneration.
- Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat mo…
- Autologous, Non-Invasively Available Mesenchymal Stem Cells from the Outer Root Sheath of Hair Folli…
- VEGF165 induces differentiation of hair follicle stem cells into endothelial cells and plays a role …
- Human hair-follicle associated pluripotent (hHAP) stem cells differentiate to cardiac-muscle cells.
- The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Line…
- Hair-follicle-associated pluripotent stem cells derived from cryopreserved intact human hair follicl…
- Implanted hair-follicle-associated pluripotent (HAP) stem cells encapsulated in polyvinylidene fluor…
- CD34 defines melanocyte stem cell subpopulations with distinct regenerative properties.
- In vitro neural differentiation of CD34 (+) stem cell populations in hair follicles by three differe…
같은 제1저자의 인용 많은 논문 (5)
- Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves.
- Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function.
- Nascent blood vessels in the skin arise from nestin-expressing hair-follicle cells.
- Human hair follicle pluripotent stem (hfPS) cells promote regeneration of peripheral-nerve injury: an advantageous alternative to ES and iPS cells.
- Direct transplantation of uncultured hair-follicle pluripotent stem (hfPS) cells promotes the recovery of peripheral nerve injury.