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Minoxidil sulfate is the active metabolite that stimulates hair follicles.

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The Journal of investigative dermatology 📖 저널 OA 68% 2021: 20/22 OA 2022: 11/14 OA 2023: 14/29 OA 2024: 15/36 OA 2025: 37/70 OA 2026: 32/69 OA 2021~2026 1990 Vol.95(5) p. 553-7 피인용 16회 cited 196 RCR 5.26 Hair Growth and Disorders
TL;DR These studies show that sulfation is a critical step for hair-growth effects of minoxidil and that it is the sulfated metabolite that directly affects hair follicles.
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PubMed DOI OpenAlex Semantic 마지막 보강 2026-05-10
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OpenAlex 토픽 · Hair Growth and Disorders Skin and Cellular Biology Research Dermatology and Skin Diseases

Buhl AE, Waldon DJ, Baker CA, Johnson GA

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Abstract

An important step in understanding minoxidil's mechanism of action on hair follicles was to determine the drug's active form. We used organ-cultured vibrissa follicles to test whether it is minoxidil or its sulfated metabolite, minoxidil sulfate, that stimulates hair growth. Follicles from neonatal mice were cultured with or without drugs and effects were assessed by measuring incorporation of radiolabeled cysteine in hair shafts of the treated follicles. Assays of minoxidil sulfotransferase activity indicated that vibrissae follicles metabolize minoxidil to minoxidil sulfate. Dose-response studies showed that minoxidil sulfate is 14 times more potent than minoxidil in stimulating cysteine incorporation in cultured follicles. Three drugs that block production of intrafollicular minoxidil sulfate were tested for their effects on drug-induced hair growth. Diethylcarbamazine proved to be a noncompetitive inhibitor of sulfotransferase and prevented hair growth stimulation by minoxidil but not by minoxidil sulfate. Inhibiting the formation of intracellular PAPS with chlorate also blocked the action of minoxidil but not of minoxidil sulfate. Acetaminophen, a potent sulfate scavenger blocked cysteine incorporation by minoxidil. It also blocked follicular stimulation by minoxidil sulfate apparently by directly removing the sulfate from the drug. Experiments with U-51,607, a potent minoxidil analog that also forms a sulfated metabolite, showed that its activity was inhibited by both chlorate and diethylcarbamazine. These studies show that sulfation is a critical step for hair-growth effects of minoxidil and that it is the sulfated metabolite that directly affects hair follicles.
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These studies show that sulfation is a critical step for hair-growth effects of minoxidil and that it is the sulfated metabolite that directly affects hair follicles.

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APA 7 Buhl, A. E., Waldon, D. J., Baker, C. A., & Johnson, G. A. (1990). Minoxidil sulfate is the active metabolite that stimulates hair follicles.. The Journal of investigative dermatology, 95(5), 553-7. https://doi.org/10.1111/1523-1747.ep12504905
Vancouver Buhl AE, Waldon DJ, Baker CA, Johnson GA. Minoxidil sulfate is the active metabolite that stimulates hair follicles. Jour. inve. derm.. 1990;95(5):553-7. doi:10.1111/1523-1747.ep12504905
AMA 11 Buhl AE, Waldon DJ, Baker CA, Johnson GA. Minoxidil sulfate is the active metabolite that stimulates hair follicles. Jour. inve. derm.. 1990;95(5):553-7. doi:10.1111/1523-1747.ep12504905
Chicago Buhl, A. E., Waldon, D. J., Baker, C. A., and Johnson, G. A.. 1990. "Minoxidil sulfate is the active metabolite that stimulates hair follicles." The Journal of investigative dermatology 95 (5): 553-7. https://doi.org/10.1111/1523-1747.ep12504905
MLA 9 Buhl, A. E., et al. "Minoxidil sulfate is the active metabolite that stimulates hair follicles." The Journal of investigative dermatology, vol. 95, no. 5, 1990, pp. 553-7. doi:10.1111/1523-1747.ep12504905.
PMID 2230218 ↗

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