Platelet-rich plasma in androgenic alopecia: myth or an effective tool.
4/5 보강
TL;DR
PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction, according to this prospective study.
PICO 자동 추출 (휴리스틱, conf 2/4)
유사 논문P · Population 대상 환자/모집단
9 patients.
I · Intervention 중재 / 시술
추출되지 않음
C · Comparison 대조 / 비교
추출되지 않음
O · Outcome 결과 / 결론
Therefore, average mean gain is 22.09 follicular units per cm(2.) After the fourth session, the pull test was negative in 9 patients. [CONCLUSION] PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction.
📑 코퍼스 인용 관계
· 인용됨 35
📑 인용한 논문 (6) ▾
- Comparative Efficacy and Safety of Platelet Rich Plasma (PRP) versus Topical Minoxidil for… Aesthetic plastic surgery · 2026
- Kopexil vs minoxidil: comparative study on hair growth, hair growth promoting factors and… BioImpacts : BI · 2025
- Efficiency of 5% Minoxidil and Platelet-rich Plasma Therapy in Androgenetic Alopecia - A R… Annals of maxillofacial surgery · 2025
- Inflammatory Signatures and Biological Markers in Platelet-Rich Plasma Therapy for Hair Re… Diagnostics (Basel, Switzerland) · 2025
- Effectiveness of Autologous Platelet-Rich Plasma for Androgenetic Alopecia: A Double-Cente… Clinical, cosmetic and investigational dermatology · 2025
- Evaluation of Role and Efficacy of Autologous Platelet-Rich Plasma Injection Treatment for… Journal of pharmacy & bioallied sciences · 2025
연도별 인용 (2015–2025) · 합계 169
OpenAlex 토픽 ·
Hair Growth and Disorders
Facial Rejuvenation and Surgery Techniques
Periodontal Regeneration and Treatments
Abstract 🌐 Abstract
[UNLABELLED] Platelet-rich plasma (PRP) has become a newer method for the treatment of various types of alopecia. In this prospective study, safety, efficacy and feasibility of PRP injections in treating androgenic alopecia were assessed. Eleven patients suffering from hair loss due to androgenic alopecia and not responding to 6 months treatment with minoxidil and finasteride were included in this study. The hair pull test was performed before every treatment session. A total volume of 2-3 cc PRP was injected in the scalp by using an insulin syringe. The treatment was repeated every two weeks, for a total of four times. The outcome was assessed after 3 months by clinical examination, macroscopic photos, hair pull test and patient's overall satisfaction.
[RESULTS] A significant reduction in hair loss was observed between first and fourth injection. Hair count increased from average number of 71 hair follicular units to 93 hair follicular units. Therefore, average mean gain is 22.09 follicular units per cm(2.) After the fourth session, the pull test was negative in 9 patients.
[CONCLUSION] PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction.
[RESULTS] A significant reduction in hair loss was observed between first and fourth injection. Hair count increased from average number of 71 hair follicular units to 93 hair follicular units. Therefore, average mean gain is 22.09 follicular units per cm(2.) After the fourth session, the pull test was negative in 9 patients.
[CONCLUSION] PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction.
PRP injection is a simple, cost effective and feasible treatment option for androgenic alopecia, with high overall patient satisfaction, according to this prospective study.
APA 7
Khatu, S. S., More, Y. E., Gokhale, N. R., Chavhan, D. C., & Bendsure, N. (2014). Platelet-rich plasma in androgenic alopecia: Myth or an effective tool.. Journal of cutaneous and aesthetic surgery, 7(2), 107-10. https://doi.org/10.4103/0974-2077.138352
Vancouver
Khatu SS, More YE, Gokhale NR, Chavhan DC, Bendsure N. Platelet-rich plasma in androgenic alopecia: myth or an effective tool. Jour. cuta. aest. surg.. 2014;7(2):107-10. doi:10.4103/0974-2077.138352
AMA 11
Khatu SS, More YE, Gokhale NR, Chavhan DC, Bendsure N. Platelet-rich plasma in androgenic alopecia: myth or an effective tool. Jour. cuta. aest. surg.. 2014;7(2):107-10. doi:10.4103/0974-2077.138352
Chicago
Khatu, S. S., More, Y. E., Gokhale, N. R., Chavhan, D. C., and Bendsure, N.. 2014. "Platelet-rich plasma in androgenic alopecia: myth or an effective tool." Journal of cutaneous and aesthetic surgery 7 (2): 107-10. https://doi.org/10.4103/0974-2077.138352
MLA 9
Khatu, S. S., et al. "Platelet-rich plasma in androgenic alopecia: myth or an effective tool." Journal of cutaneous and aesthetic surgery, vol. 7, no. 2, 2014, pp. 107-10. doi:10.4103/0974-2077.138352.
PMID
25136212 ↗
🏷️ 키워드 / MeSH 📖 같은 키워드 OA만
인용 관계
그래프 OA 노드: 5/8 (63%)
· 참조 0편 · 후속 5편
이 논문을 인용한 후속 연구 20
- The Effect of Platelet-Rich Plasma in Hair Regrowth: A Randomized Placebo-Controlled Trial.
- Androgenetic Alopecia: An Update of Treatment Options.
- Alopecia areata: a review on diagnosis, immunological etiopathogenesis and treatment options.
- Systematic Review of Platelet-Rich Plasma Use in Androgenetic Alopecia Compared with Minoxidil, Fina…
- A systematic summary of survival and death signalling during the life of hair follicle stem cells.
- Alopecia Areata: Current Treatments and New Directions.
- Comparative Evaluation of the Clinical Efficacy of PRP-Therapy, Minoxidil, and Their Combination wit…
- A Study to Compare the Efficacy of Platelet-rich Plasma and Minoxidil Therapy for the Treatment of A…
- Effectiveness of Platelet-Rich Plasma for Androgenetic Alopecia: A Review of the Literature.
- Clinical and preclinical approach in AGA treatment: a review of current and new therapies in the reg…
- A Comparative Study of Therapeutic Response to Intralesional Injections of Platelet-Rich Plasma Vers…
- A Systemic Review on Topical Marketed Formulations, Natural Products, and Oral Supplements to Preven…
- Platelet-rich Plasma for Androgenetic Alopecia Treatment: A Randomized Placebo-controlled Pilot Stud…
- Efficacy of Platelet-Rich Plasma and Concentrated Growth Factor in Treating Androgenetic Alopecia - …
- A Meta-analysis On Evidence Of Platelet-rich Plasma for Androgenetic Alopecia.
- Platelet-rich plasma-an 'Elixir' for treatment of alopecia: personal experience on 117 patients with…
- Human Hair Outer Root Sheath Cells and Platelet-Lysis Exosomes Promote Hair Inductivity of Dermal Pa…
- Injectable-Platelet-Rich Fibrin-Smart Blood with Stem Cells for the Treatment of Alopecia: A Report …
- Hair Transplant Practice Guidelines.
- Dermoscopic Assessment of Microneedling Alone versus Microneedling with Platelet-Rich Plasma in Case…
📖 전문 본문 읽기 PMC JATS · ~11 KB · 영문
INTRODUCTION
INTRODUCTION
Androgenic alopecia or male pattern baldness is a very common type of hair loss observed in both males and females. Platelet-rich plasma (PRP) is an autologous preparation of platelets in concentrated plasma. Although the optimal PRP platelet concentration is unclear, the current methods by which PRP is prepared report 300-700% enrichment, with platelet concentrations consequently increasing to more than 1,000,000 platelets/L.[1] PRP has attracted attention in several medical fields because of its ability to promote wound healing. Activation of alpha granules of platelets releases numerous proteins, including platelet-derived growth factor (PDGF), transforming growth factor (TGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF) and interleukin (IL)-1.[23] It is hypothesised that growth factors released from platelets may act on stem cells in the bulge area of the follicles, stimulating the development of new follicles and promoting neovascularisation.[4] The aim our pilot study was to evaluate the safety, efficacy and feasibility of PRP for treatment of androgenic alopecia.
Androgenic alopecia or male pattern baldness is a very common type of hair loss observed in both males and females. Platelet-rich plasma (PRP) is an autologous preparation of platelets in concentrated plasma. Although the optimal PRP platelet concentration is unclear, the current methods by which PRP is prepared report 300-700% enrichment, with platelet concentrations consequently increasing to more than 1,000,000 platelets/L.[1] PRP has attracted attention in several medical fields because of its ability to promote wound healing. Activation of alpha granules of platelets releases numerous proteins, including platelet-derived growth factor (PDGF), transforming growth factor (TGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF) and interleukin (IL)-1.[23] It is hypothesised that growth factors released from platelets may act on stem cells in the bulge area of the follicles, stimulating the development of new follicles and promoting neovascularisation.[4] The aim our pilot study was to evaluate the safety, efficacy and feasibility of PRP for treatment of androgenic alopecia.
MATERIALS AND METHODS
MATERIALS AND METHODS
Between August 2013 and November 2013, all patients suffering from androgenic alopecia and on topical minoxidil and finasteride for at least 6 months without much improvement were considered for PRP therapy. Written informed consent was obtained. All included patients were tested by ELISA for HIV, HBS Ag and platelet count. Exclusion criteria were haematological disorders, thyroid dysfunction, malnutrition and other dermatological disorders contributing to hair loss. A 1 cm × 1 cm square area was marked over right parietal area in mid-pupillary line, 10 cm proximal to right eyebrow in each patient. Baseline follicular units were manually counted with the help of trichoscan in this area by dividing into four small quadrants [Figures 1 and 2].
Before each session, the hair pull test was performed three times by the same clinician. All patients were advised to avoid washing hair two days prior to the treatment. A bundle of approximately 50-60 hair was grasped between the thumb, index and middle finger from the base close to the scalp. The hair was firmly tugged away from the scalp, and the extracted hair was counted in every session. To evaluate overall hair growth, hair volume, hair quality and fullness, global pictures were taken in every session from front, vertex, lateral and back view.
PRP was prepared by collecting 20 cc of fresh blood in sodium citrate containing vaccutainers in minor operation theatre under proper aseptic precaution. The tubes were rotated in a centrifugation machine at 1500 revolutions per minute for 6 minutes. The first centrifugation is called “soft spin”, which allows blood separation into three layers, namely bottom RBC layer (55% of total volume), topmost acellular plasma layer called platelet poor plasma (PPP, 40% of total volume) and an intermediate PRP layer (5% of total volume) called the “buffy coat”. Separated buffy coat with PPP was collected with the help of Finn pipette in another test tube. This tube underwent a second centrifugation, which was longer and faster than the first, called “hard spin”, comprising at 2500 revolution per minute for 15 minutes. This allows the platelets (PRP) to settle at the bottom of the tube. The upper layer containing PPP was discarded and the lower layer of PRP was loaded in an insulin syringe containing calcium chloride (1 part calcium chloride and 9 parts of PRP) as an activator.[5]
One hour prior to administration of PRP, anaesthetic cream was applied over the bald area. Area of the scalp to be treated was cleaned with cetavlon, spirit and povidone-iodine. With the help of insulin syringe PRP was injected over affected area by nappage technique (multiple small injections in a linear pattern one-cm apart) under proper aseptic precaution in minor operation theatre. A total volume of 2-3 cc was injected. The treatment was repeated every two weeks for four sessions. At each visit, hair count was noted over the prefixed square area. Subjective improvements of patients were noted on a scale of worst (1) to best (10). We evaluated all the patients at the end of 12 weeks.
Between August 2013 and November 2013, all patients suffering from androgenic alopecia and on topical minoxidil and finasteride for at least 6 months without much improvement were considered for PRP therapy. Written informed consent was obtained. All included patients were tested by ELISA for HIV, HBS Ag and platelet count. Exclusion criteria were haematological disorders, thyroid dysfunction, malnutrition and other dermatological disorders contributing to hair loss. A 1 cm × 1 cm square area was marked over right parietal area in mid-pupillary line, 10 cm proximal to right eyebrow in each patient. Baseline follicular units were manually counted with the help of trichoscan in this area by dividing into four small quadrants [Figures 1 and 2].
Before each session, the hair pull test was performed three times by the same clinician. All patients were advised to avoid washing hair two days prior to the treatment. A bundle of approximately 50-60 hair was grasped between the thumb, index and middle finger from the base close to the scalp. The hair was firmly tugged away from the scalp, and the extracted hair was counted in every session. To evaluate overall hair growth, hair volume, hair quality and fullness, global pictures were taken in every session from front, vertex, lateral and back view.
PRP was prepared by collecting 20 cc of fresh blood in sodium citrate containing vaccutainers in minor operation theatre under proper aseptic precaution. The tubes were rotated in a centrifugation machine at 1500 revolutions per minute for 6 minutes. The first centrifugation is called “soft spin”, which allows blood separation into three layers, namely bottom RBC layer (55% of total volume), topmost acellular plasma layer called platelet poor plasma (PPP, 40% of total volume) and an intermediate PRP layer (5% of total volume) called the “buffy coat”. Separated buffy coat with PPP was collected with the help of Finn pipette in another test tube. This tube underwent a second centrifugation, which was longer and faster than the first, called “hard spin”, comprising at 2500 revolution per minute for 15 minutes. This allows the platelets (PRP) to settle at the bottom of the tube. The upper layer containing PPP was discarded and the lower layer of PRP was loaded in an insulin syringe containing calcium chloride (1 part calcium chloride and 9 parts of PRP) as an activator.[5]
One hour prior to administration of PRP, anaesthetic cream was applied over the bald area. Area of the scalp to be treated was cleaned with cetavlon, spirit and povidone-iodine. With the help of insulin syringe PRP was injected over affected area by nappage technique (multiple small injections in a linear pattern one-cm apart) under proper aseptic precaution in minor operation theatre. A total volume of 2-3 cc was injected. The treatment was repeated every two weeks for four sessions. At each visit, hair count was noted over the prefixed square area. Subjective improvements of patients were noted on a scale of worst (1) to best (10). We evaluated all the patients at the end of 12 weeks.
RESULTS
RESULTS
Eleven male patients in the age group of 20-40 years were included in the study. According to Hamilton classification of male pattern baldness, 4 patients were in grade 2, 4 patients were in grade 3 and 3 patients were in grade 4 androgenic alopecia.
Before treatment, all our patients (100%) had a positive hair pull test with mean number of 10 hair. After the fourth session, the pull test was negative in 9 patients (81.81%) with average number of three hairs. A significant reduction in hair loss was observed between first and fourth injection as noticed by patients. Global pictures also revealed a moderate improvement in hair volume and coverage. [Figures 3 and 4] Hair count depicted average number of 71 hair follicular units over marked area before starting the treatment, and after 4 sessions of PRP, average number of follicular units was 93.09 follicular units. Therefore, average mean gain is 22.09 follicular units per cm2. Overall, patient satisfaction was high with a mean result rating of 7.0 on a scale of 1-10.
The side effects after PRP injections were minimal pain, redness at the time of injections and pinpoint bleeding.
Eleven male patients in the age group of 20-40 years were included in the study. According to Hamilton classification of male pattern baldness, 4 patients were in grade 2, 4 patients were in grade 3 and 3 patients were in grade 4 androgenic alopecia.
Before treatment, all our patients (100%) had a positive hair pull test with mean number of 10 hair. After the fourth session, the pull test was negative in 9 patients (81.81%) with average number of three hairs. A significant reduction in hair loss was observed between first and fourth injection as noticed by patients. Global pictures also revealed a moderate improvement in hair volume and coverage. [Figures 3 and 4] Hair count depicted average number of 71 hair follicular units over marked area before starting the treatment, and after 4 sessions of PRP, average number of follicular units was 93.09 follicular units. Therefore, average mean gain is 22.09 follicular units per cm2. Overall, patient satisfaction was high with a mean result rating of 7.0 on a scale of 1-10.
The side effects after PRP injections were minimal pain, redness at the time of injections and pinpoint bleeding.
DISCUSSION
DISCUSSION
Hair loss has a significant influence on psychological distress and is associated with low self-esteem and depression. Treatment options for androgenic alopecia are very limited and include topical minoxidil and oral finasteride (FDA approved) either alone or in combination.[67] However, there are several reported side effects such as headache and increase in other body hairs for minoxidil[6] whereas loss of libido has been reported with oral finasteride. Finasteride also interferes with genital development in a male fetus and is contraindicated in pregnant women and those likely to become pregnant.[78]
PRP has already attracted attention in plastic surgery, orthopaedic surgery and cardiac surgery because of its potential use in skin rejuvenating effects, rapid healing, reduced infection, decreased chance of hypertrophic keloids and scars.[910] Growth factors are known to activate the proliferative phase and transdifferentiation of hair and stem cells and produce new follicular units. bFGF is reported to promote the in vitro proliferation of papilla cells, and thereby plays a key role in elongating hair shaft.[11]
We prepared PRP by double spin method, in which blood cell layers were manually separated. Activation of platelets through coagulation triggers the secretion of various growth factors, which produce mitogenic effects in various cell types. Activated PRP promotes the proliferation of dermal papillary cells and prevents their apoptosis.
In our study, the hair pull test became negative after four sessions of PRP. This finding is comparable with the study conducted by Besti et al.[12] This study also observed significant improvement in hair volume and coverage in global pictures, but according to our study, only moderate improvement in hair volume and coverage was observed.[12]
Uebel et al. observed a significant improvement in hair density and stimulation of growth when follicular units were pre-treated with platelet plasma growth factors before their implantation. There was a significant difference in the yield of follicular units on comparing the experimental with the control areas of the scalp. The areas treated with platelet plasma growth factors demonstrated a yield of 18.7 follicular units per cm2 whereas the control areas yielded 16.4 follicular units per cm2, an increase in follicular density of 15.1 percent.[4]
Our study has some limitations. Trichoscopic hair evaluation could have given more objective results. Sample size is very small. Mean follow up of patients is also short to draw conclusion regarding the long-term effectiveness of treatment. Thus, further studies are needed with longer follow up and with large sample size.
Hair loss has a significant influence on psychological distress and is associated with low self-esteem and depression. Treatment options for androgenic alopecia are very limited and include topical minoxidil and oral finasteride (FDA approved) either alone or in combination.[67] However, there are several reported side effects such as headache and increase in other body hairs for minoxidil[6] whereas loss of libido has been reported with oral finasteride. Finasteride also interferes with genital development in a male fetus and is contraindicated in pregnant women and those likely to become pregnant.[78]
PRP has already attracted attention in plastic surgery, orthopaedic surgery and cardiac surgery because of its potential use in skin rejuvenating effects, rapid healing, reduced infection, decreased chance of hypertrophic keloids and scars.[910] Growth factors are known to activate the proliferative phase and transdifferentiation of hair and stem cells and produce new follicular units. bFGF is reported to promote the in vitro proliferation of papilla cells, and thereby plays a key role in elongating hair shaft.[11]
We prepared PRP by double spin method, in which blood cell layers were manually separated. Activation of platelets through coagulation triggers the secretion of various growth factors, which produce mitogenic effects in various cell types. Activated PRP promotes the proliferation of dermal papillary cells and prevents their apoptosis.
In our study, the hair pull test became negative after four sessions of PRP. This finding is comparable with the study conducted by Besti et al.[12] This study also observed significant improvement in hair volume and coverage in global pictures, but according to our study, only moderate improvement in hair volume and coverage was observed.[12]
Uebel et al. observed a significant improvement in hair density and stimulation of growth when follicular units were pre-treated with platelet plasma growth factors before their implantation. There was a significant difference in the yield of follicular units on comparing the experimental with the control areas of the scalp. The areas treated with platelet plasma growth factors demonstrated a yield of 18.7 follicular units per cm2 whereas the control areas yielded 16.4 follicular units per cm2, an increase in follicular density of 15.1 percent.[4]
Our study has some limitations. Trichoscopic hair evaluation could have given more objective results. Sample size is very small. Mean follow up of patients is also short to draw conclusion regarding the long-term effectiveness of treatment. Thus, further studies are needed with longer follow up and with large sample size.
CONCLUSION
CONCLUSION
PRP injection for androgenic alopecia is a simple, cost-effective and feasible treatment option for hair loss and can be regarded as a valuable adjuvant treatment modality for androgenic alopecia. Although PRP has sufficient theoretical scientific basis to support its use in hair restoration, hair restoration using PRP is still at its infancy. Clinical evidence is still weak. Considering its excellent safety profile and relatively low cost, PRP hair treatment is a promising treatment option for patients with thinning hair.
PRP injection for androgenic alopecia is a simple, cost-effective and feasible treatment option for hair loss and can be regarded as a valuable adjuvant treatment modality for androgenic alopecia. Although PRP has sufficient theoretical scientific basis to support its use in hair restoration, hair restoration using PRP is still at its infancy. Clinical evidence is still weak. Considering its excellent safety profile and relatively low cost, PRP hair treatment is a promising treatment option for patients with thinning hair.
출처: PubMed Central (JATS). 라이선스는 원 publisher 정책을 따릅니다 — 인용 시 원문을 표기해 주세요.
🏷️ 같은 키워드 · 무료전문 — 이 논문 MeSH/keyword 기반
- 패턴형 탈모의 분류: 고찰.
- 탈모 치료에서 비활성화 및 활성화 PRP의 평가: 다양한 수집 시스템으로 얻은 성장인자와 사이토카인 농도의 역할.
- 혈소판 풍부 혈장과 자가 인간 모낭 중간엽 줄기세포가 농축된 마이크로그래프트가 안드로겐성 탈모에서 모발 재성장을 향상시킨다. 생체분자 경로 분석 및 임상적 평가.
- 탈모 치료제 개발: 오늘은 사라져도 내일은 모발이.
- Quercetin과 아연/구리 이중 도핑 메조포러스 실리카 나노복합체 마이크로니들 패치를 이용한 안드로겐성 탈모 병용요법.
- 안드로겐성 탈모 치료에서 platelet-rich plasma의 효능.